Journal of Physical Chemistry & Biophysics
Open Access

Abstract

On the Use of MCLA for Chemiluminescent Measurement of Reactive Oxygen Species

Vekshin NL, Frolova MS

Verification on the using of 2-methyl-6- (4-methoxyphenyl) -3,7-dihydroimidazo [1,2-a] pyrazin-3(7H)-one (MCLA) as a chemi-luminescence (CL) sensitizer for detection of reactive oxygen species (ROS) was done. In model experiments on aqueous solutions of MCLA (in the absence of cells), it was shown that its CL is quenched by a number of substances, normally contained in cells (iron ions, glutathione, ascorbate, oxygen, protons, etc.). It has been shown that CL of MCLA in water is enhanced by sodium azide, which is often used to block the mitochondrial respiratory chain. The data obtained should be taken into account when measuring of CL from MCLA or its analogs in cells will be done. A high sensitivity of CL of MCLA to oxygen, pH, ascorbate, glutathion and iron ions could allow use MCLA to detect these substances in solutions.

Published Date: 2019-12-08; Received Date: 2019-10-22