Journal of Molecular Imaging & Dynamics
Open Access

Abstract

Johansen Michael*

It's difficult to deny that live-cell imaging hasn't impacted our
perspective on biology. Over the last ten years, there has been a
surge in interest in imaging cellular activities at the molecular
level. Many innovative approaches are now being used in live cell
imaging. Cellular health, on the other hand, is frequently
overlooked. For many researchers, all is well if the cell does not
go into apoptosis or is blabbed beyond recognition at the end of
the experiment. This is completely false. When performing livecell
imaging, numerous aspects must be considered in order to
maintain cellular health, including imaging modalities, medium,
temperature, humidity, PH, osmolality, and photon dose. Two of
the most essential and controllable aspects of live-cell imaging
are the wavelength of illuminating light and the total photon
dose that the cells are subjected to. The lowest photon dose that
yields a metric for the experimental inquiry, rather than the dose
that provides cover photo grade photos, should be employed.
This is critical to guarantee that the biological processes being
studied are in their in vitro condition and have not been
switched to a different pathway as a result of environmental
stress. The timing of the mitosis is an ideal canary in the gold
mine, in that any stress induced from the imaging will result in
the increased length of mitosis, thus providing a control model
for the current imagining conditions.

Published Date: 2021-11-17;