Journal of Applied Pharmacy
Open Access

Abstract

Investigation of Antioxidant and Cytotoxic Activity of Methanol Extracts of Oroxylum indicum and Begonia roxburghii

Dr. Raushanara Akter, Rakhi Chowdhury

Oroxylum indicum (O. indicum) and Begonia roxburghii (B. roxburghii) are medicinal plants widely used as drugs or component of drugs for treatment of various diseases. In this study, the methanol extracts of the leaf and stem of both plants were evaluated for their phytochemicals, antioxidant and cytotoxic activities. To measure in-vitro antioxidant potential, six methods were used such as DPPH free radical scavenging assay, superoxide radical scavenging activity, total phenolic content, total flavonoid content, total antioxidant content, and total antioxidant capacity determination assays. O. indicum stem and B. roxburghii leaves, showed strong antioxidant potential with IC50 values of 19.66 and 22.35 µg/mL, respectively in DPPH test and high superoxide radical scavenging activity with IC50 values of 19.57 and 26.96 µg/mL, respectively in SRS test. Moreover, both extracts showed 98.75 and 100.58 TPC (GAE) mg of gallic acid per gram of dried extract. At the highest concentration, in TAC B. roxburghii extract produced strong antioxidant potential represented by 98.58 mg of ascorbic acid per gram of dried extract whereas TACT of O. indicum found to be very high (150.56 mM of FeSO4 per gram of dried extract). In-vitro cytotoxic potential of both extracts by MTT assay at different concentrations against MOLT-4 & HeLa cell lines respectively revealed that, at the highest concentration of 25 mg/mL, leaf extracts of both plants exhibited 90.58% and 80.66% of the cell death. Based on these preliminary findings of this study, further work could be the isolation of pure compounds those may be used as drugs for treating cancer and diseases caused by free radicals.

Keywords: DPPH scavenging; TPC; TFC; TAC; SRS; TACT; MTT assay

Published Date: 2021-03-25; Received Date: 2020-09-09