Journal of Glycomics & Lipidomics
Open Access

Abstract

Investigating differences in phenotype among 9G8, RSF1, and TRN KD flies and suspected antagonism for TRN SR transport shuttle in lipid storage

Ben Borokhovsky

The method of gene regulation underlying lipid storage related to obesity is poorly understood, yet alternative splicing (AS) appears to be an important mechanism for proper lipid storage. CPT1 (carnitine palmitoyltransferase 1) is a beta-oxidation enzyme involved in the breakdown of fatty acids. The gene coding for CPT1 is alternatively spliced by the SR protein 9G8 to produce two different products that vary in their activity. My project was to investigate if there was a difference in the AS phenotype among flies with decreased expression of 9G8, the SR protein antagonist RSF1, and the SR protein transporter TRN. First, a quantitative PCR (qPCR) protocol needed to be developed to detect the isoforms of CPT1. cDNA was generated from wild type flies that were fed (0 hrs), starved (65 hrs) or refed to optimize the likelihood that both isoforms would be produced. Following a published protocol, a qPCR procedure was carried out that skipped the elongation step (73

Published Date: 2021-07-25; Received Date: 2020-11-20