Journal of Nanomedicine & Biotherapeutic Discovery
Open Access

Abstract

Inhibition of Hepatocellular Carcinoma Cell Proliferation and Metastasis by Levorotatory Polylactic Acid Electrostatically Spun Nanofibers Loaded with Ibuprofen and Acid-Sensitive Adriamycin Hydrochloride Mesoporous Silica

Yanxu Li,Weiben Ji, Zhuoheng Liu, Lai Chang, Huiyuan Hu, Kunlin Wu, Ke Wang, Chaoying, Wang, Quan Zhang, Jiefeng Gao, Tao Wang, Wei W

Background: Electrostatically spun nanocarrier systems are widely used in the medical field. Adriamycin (DOX), Sodium Bicarbonate (SB) and Ibuprofen (IBU) can inhibit tumor cell proliferation and metastasis, neutralize the acidic environment and inhibit inflammation, respectively. The drug carrier system using nanofibrous membranes can inhibit the proliferation and metastasis of tumor cells. Materials and methods: MSN was used to load the antitumor drug DOX and the composite fiber membrane was prepared by using SB to complete the acid sensitization and then synergistically dispersed into a PLLA electrostatic spinning solution with IBU. Transmission electron microscopy and scanning electron microscopy were used to characterize the composite fiber membrane. The CCK-8 assay and the apoptosis assay were used to verify the toxic effects of DOX, IBU and SB on normal cells and the apoptotic effects on tumor cells. Cell proliferation-related factors, immune/inflammatory factors and apoptosis-related factors were detected by Western blot, ELISA and qPCR. Results: DOX decreased its own cytotoxicity after being loaded by MSN and wrapped by PLLA. The inhibitory and pro-apoptotic effects on tumor cell invasion were not significantly reduced and there was no obvious toxic effect on normal cells. The nanomaterials themselves could promote the elevation of the ROS level in tumor cells and the IBUs could attenuate the expression of inflammatory factors. The nanofiber membrane can promote apoptosis of HCC cells and inhibit the proliferation and metastasis of HCC cells under the combined effect. Conclusion: Poly (L-lactic acid) electrostatically spun nanofibers loaded with ibuprofen and acid-sensitive adriamycin hydrochloride-loaded mesoporous silica showed significant cytotoxicity to tumor cells and no significant toxic effects on normal cells.

Published Date: 2025-03-05;