Numerous kind of antioxidants or protecting agent are present in the living body like Glutathione is the master antioxidant produce by the liver and uses free radicals to purify the body. Natural plant consuming antioxidants content is growing the interest for scientific research as well as industrial purposes. Pteridophytes (fern and fern allies) have drawn attention of plants seekers and horticultures since ancient period. Plant of Pteris vittata were collected from different geographical region of Uttarakhand Garhwal. The maximum yield of plant extract was found via the ethanol solvent i.e. 5.07-9.67%. DPV extract exhibited a maximum inhibition of 89.32 relatively closed to 91.96% inhibition of Ascorbic acid at the concentration of 0.1 mg/ml by DPPH radical scavenging assay method. The IC50 of the DPV extract and Ascorbic acid was found to be 0.543 and 0.495 mg/ml by same. DPV extract showed a maximum inhibition of 72.33 % relatively close to 77.42% inhibition of BHA at the concentration of 0.8 mg/ml by Hydrogen peroxide radical scavenging method. The IC50 value of the DPV extract & BHA was found to be 0.279 ± 0.005 mg/ml & 0.257 ± 0.002 mg/ml by same. DPV extract showed a maximum inhibition of 84.32% relatively close to 87.96% inhibition of ascorbic acid at the concentration of 0.8 mg/ml by Nitrogen oxide scavenging method. The IC50 value of the DPV extract & BHA was found to be 0.233 ± 0.002 mg/ml to 0.218 ± 0.006 mg/ml by same.