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Suleiman MS, Nuntah LC, Muhammad HL, Mailafiya SC, Makun HA, Saidu AN, Apeh DO and Iheanacho HE
Mycoflora and aflatoxin contamination was determined in 200 samples of fresh and dried vegetable, which are widely used because of their nutritional and medicinal principles. Fifty (50) fresh pumpkin (Curcurbita spp), fresh spinach (Spinancia oleracea), bitter leaf (Vernonia amygdalina) comprising of (25 dried and 25 fresh) and 50 samples of tomato (Solanum lycopersicum) (25 dried and 25 fresh) were collected from local vegetable vendors in Minna metropolis, Nigeria for investigation. Identification of isolates was carried out based on their morphological and microscopic characteristics. A total of 165 isolates made up of 4 genera (Aspergillus, Penicillum, Fusarium and Mucor) were identified. Aflatoxins (AF) were determined using High Performance Liquid Chromatography technique with UV detection. The dried bitter leaf samples was more (P<0.05) susceptible to aflatoxin contamination than fresh bitter leaf and pumpkin leaf samples. Fifty two percent (52%) of all aflatoxins contamination was by AFG1, no significant (P>0.05) difference was observed between AFB1 (40%) and AFB2 (44%) in the dried bitter leaf samples. Aflatoxins were detected in tomato (fresh and dried) and spinach samples at lower concentrations (0.00-9.12 μg/kg). This report calls for a need for enlightenment on proper agricultural, storage and hygienic practices alongside the hazards related with aflatoxin-contaminated foods.