A new series of 2-(4-substituted phenyl)-5-(6-substituted-2-chloroquinolin-3-yl)-1,3,4oxadiazole derivatives were synthesized after refluxing 6-substituted-2-hydroxyquinolin-3-carboxylic acids with different aromatic acid hydrazides in the presence of POCl3. The chemical structures of these compounds were confirmed by various physico-chemical methods viz. IR, 1H-NMR, EI-Mass, C13-NMR and elemental analysis. Newly synthesized compounds were screened in vitro for their cytotoxic activity against SK-2-MEL cell line. Also, screened in vitro for their antimicrobial activity against varieties of gram-positive and gram-negative bacterial strains and fungi strains Aspergillus niger and Rhizopus. The compound 7a and 7e shows highly significant antimicrobial and compound 7i shows good cytotoxic activity as compared to the standard drug.