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Objective: In order to make such a substance cross the epidermal barrier, was utilized a method of transdermal drug delivery system (TDDS) combined with the chemotherapeutic etoposide in the treatment of dorsal melanoma B16F10 tumor on C57BL/6J mice.
Methods: The treatment groups were as follows: etoposide followed by radiofrequency (RF); RF followed by etoposide; etoposide and controls. The animals were treated with delivery interval of 72 hours, for 20 days and were analyzed the tumor growth; weight and hematological profile. The histological analysis and cell cycle by flow cytometry were performed after end of the treatment period.
Results: The tumors treated with RF followed by etoposide showed slower growth compared to the tumors treated with etoposide followed by RF, and found that treatment with radiofrequency considerably increased the dorsal tumor growth. In fact, the increase in tumor mass is because the radiofrequency cause an inflammatory response and stimulate collagen production by fibroblasts.
Conclusions: The results showed that the treatment groups RF followed by etoposide showed a high rate sub- G1 phase cells, indicating better therapeutic efficacy. Therefore, it is important to clarify that the referred technologies are not as harmless at it has been reported.