Journal of Clinical and Cellular Immunology
Open Access

Abstract

Evaluation of Infiltration of Immunological Cells (Tissue Eosinophil and Mast Cell) in Odontogenic Cysts by Using Special Stains

Priyanka Debta, Fakir Mohan Debta, Minal Chaudhary and Vijay Wadhwan

Cells of immune system comprised of lymphoid series and myeloid progenitor series cells. Mast cells and tissue eosinophils both are granulocytes which come under myeloid progenitor series of immune cells system. The presence of mast cells in odontogenic cyst could contribute to their pathogenesis in several ways. Mast cells liberate ECF (Eosinophils chemoattractant factor) and histamine, which attract eosinophils in tissue. Mast cells and eosinophils both have also been implicated in stimulating the production of prostaglandins, important in bone resorption for odontogenic cyst growth. The mast cells and eosinophils are features of both keratinizing and non-keratinizing cysts (OKC, DC, RC) but their number does not necessarily correlate with degree of inflammation. Special stains to demonstrate mast cells and eosinophils were used. Carbol Chromotrope staining method to demonstrate tissue eosinophil was found to be better than Congo red. The staining intensity for mast cells was equally good with thionin and toluidine blue.