Bacterial Removal of Lead and Mercury Elements from Water using Pseudomonas aeruginosa in vitro | Abstract

Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

+32 28087017


Bacterial Removal of Lead and Mercury Elements from Water using Pseudomonas aeruginosa in vitro

Rabie Ali Babiker*, Usama Abdalla Elsharief , Jamal Bayed Salim, Nadia Abdel Rahiam Mohammed,Hamdia Mohammed Abdallah

Background: Environmental pollution is the presence of a pollutant in the environment: air, water and soil, which may be lethal or toxic and will cause harmful to living things in the polluted environment. Thus removal of these toxic heavy metals from waste water is of crucial importance to protect the human population and the environment. The aim of the present study was to determine the capability of decreasing high concentrations of lead and mercury, in a laboratory experiment using Pseudomonas aeruginosa ATCC 27853 which was obtained from National Public Health Laboratory or The Stack Medical Laboratories. Method and results: P.aeruginosa was cultivated in Peptone water and incubated for 48 hours. Different concentrations of heavy metal (Pb2+, Hg2+) were made in 30 ml of distill water, 5 ml of cultivated peptone water was added to each concentrations of heavy metals separately, incubated for 7 days at 37°C. Colorimetric method was done using ????max (520-540) nm to detect the absorbance of heavy metal (Pb2+, Hg2+) respectively. Absorbance was read before and after cultivation. The results of absorbance before cultivation were recorded. In the table for Pb2+, and for Hg2+, the absorbance of Pb2+ after cultivation were revealed reduction which indicates removal of Pb2+ from solution and also absorbance of Hg2+ was decreased as result of removal Hg2+ from solution. The mean and Std. Deviation of concentration of (Pb2+, Hg2+) after cultivation were calculated by using SPSS. The results generally showed that Pb2+ and Hg levels were diminished in all the samples due to P. aeruginosa cultivation. Conclusion and recommendation: It was concluded that P. aeruginosa assisted in removing harmful pollutants (lead and mercury) from water by uptake and accumulation of heavy metal from the water sample. It is recommended that further studies should be carried out to get rid of bacteria.

Published Date: 2020-02-24; Received Date: 2020-02-01