The main goal of cancer therapy is to not only treat cancer but also to protect healthy cells from the toxic effect of the chemotherapeutic agent. For this purpose, different modern techniques are devised. The significant localized chemotherapeutic effect can be induced by combining all these techniques. Bacteria have been used as a novel organism for chemotherapy. Many new chemotherapeutic drug delivery techniques have come into existence these techniques involve bacterial cloning. The cloned bacteria contain either drug carrying gene or a prodrug-activating enzyme. Bacteria chosen for therapy should be able to grow in hypoxic tumor condition. Experiments done on Clostridium (anaerobic bacterium), produces tumor suppression in many animals.