Biochemistry & Pharmacology: Open Access
Open Access

Abstract

Apoptotic Effect of STAMP2 Transfection to PC3 Cells and Caspase Induction with Hydrogen Peroxide Incubation at Lncap Cells- Role of P53

Ceren Gonen-Korkmaz, Gulnur Sevin, Goksel Gokce, Mehmet Zuhuri Arun, Gunay Yetik-Anacak and Gokce Yildirim

STAMP genes STAMP1/STEAP2 and STAMP2/STEAP4 are expressed in androgen receptor positive-prostate cancer cell line LNCaP, and this regulation was reported, previously. The androgen induction was done at LNCaP cells. STAMP2/STEAP4 was silenced for 1, 2 and 5 days. Using real-time RT-PCR, an apoptosis panel including proapoptotic and/or apoptotic genes was investigated for gene expression alterations in STAMP expressing LNCaP cells in comparison to P53-null PC3 cells in which STAMP2 gene was transfected. In addition, promoter region of the STAMP2 gene was analyzed and putative p53 and androgen receptor (AR) response elements were identified. Thus, p53 gene was silenced using siRNA approach and was induced by hydrogen peroxide. Taken together, prostate-specific STAMP2 gene and its regulation with the p53- and caspase-related pathway was characterized. These results will shed light on understanding the control of proliferation in neoplastic transformation in prostate cancer.