Journal of Plant Biochemistry & Physiology

Journal of Plant Biochemistry & Physiology
Open Access

ISSN: 2329-9029

+44 1478 350008


Antioxidant Activity of Pulse Hydrocolloids: Classical Screening Methods Depending on Water Soluble Phenolic Antioxidants Need Revision to Measure True Antioxidant Potential of Pulses

Levent Yurdaer Aydemir and Ahmet Yemenicioglu

The aim of this study is to show the presence of considerable amounts of antioxidants bound on pulse hydrocolloids. For this purpose, 6 lentil and 4 chickpea cultivars were tested for their free radical scavenging based antioxidant capacities in water soluble extracts and hydrocolloid extracts. The results clearly showed that the antioxidant potential of pulses based on hydrocolloid extracts accounts for 28 to 89% of those based on soluble phenolic antioxidants. Moreover, the antioxidant activity measurements in hydrocolloid extracts help much more than those in water extracts to determine differences among the antioxidant potentials of pulse cultivars. Extensive screening studies have been conducted by plant breeders to understand the importance of different phenotypic and genetic factors as well as the growth conditions on antioxidant status of the plants. This work clearly showed that not only water extracts, but also hydrocollid extracts should be considered to conduct realistic screening studies.