Applied Microbiology: Open Access
Open Access

Abstract

Antibacterial Investigation of Silver Nanoparticle against Staphylococcus, E. coli and Salmonella Isolated from Selected Live Bird Markets

Kumar Jyotirmoy Roy, Aminur Rahman, KhandkerSaadat Hossain, Md. Bahanur Rahman, Md. AbdulKafi*

An antibiogram study of newly introduced Silver Nano particles (AgNPs) was performed against the available poultry bacteria. Herein, this research involved isolation, identification and characterization of Staphylococcus, E. coli and Salmonella of different bird types from two live bird markets surrounding Bangladesh Agricultural University (BAU) campus for determining antibiogram profiles against AgNP in comparison with commercially available antibiotic disks. For this a total of 120 cloacal swab samples collected from Broiler (48), Sonali (48) and Deshi chicken (24) from Kamal Ranjit (KR) and Kewatkhali live bird market from which Staphylococcus (68), E. coli (97) and Salmonella (91) were isolated. Representative of these three bacterial isolates were used for antibiogram profiling. The standard disk diffusion method was employed for the antibiogram assays where the zone of inhibition surrounding the disk used as a parameter for evaluating the sensitivity of AgNP as well as commercial antibiotic disk. The isolates showed sensitivity to Ceftriaxone (26.99 ± 0.2), Ciprofloxacine (21.57 ± 0.15), Gentamycin (23.59 ± 0.2) and resistance to Amoxicillin (0.00), Tetracycline (7.23 ± 0.06) while AgNP (22.93 ± 0.38) was sensitive against all the isolates. Thus AgNP proved sensitivity to bacteria even resistant to Amoxicillin and Tetracycline. This sensitivity of AgNP against poultry bacteria holds promise for the use as antibacterial in poultry industry.

Published Date: 2020-06-11; Received Date: 2020-05-21