Catalyst is a chemical molecule or metal substance which enhances the rate of reaction is called catalyst, the catalyst present in the living organisms to carryout biochemical reactions or metabolic pathways. Exames oxidoreductases, lyases, ligages, proteases, hydrolases, esterases, pectinases etc. are Different methodologies available in synthesis of chiral molecules chemical/biocatalysis. The enormous potential of biocatalysts (microorganisms and enzymes) in synthesis of chiral molecules in mild conditions (pH and temperate) with high chemo-, regio-, enantio and functional selectivity with decrease formation of by-products, with short reaction steps (reactions which are not easily conducted classical organic reactions) (Long chemical processes with tedious blocking and de-blocking steps). Thus the use of biocatalyst has attracted a great attention from the green chemistry perspective (expensive chiral reagents/ environmentally hazardous heavy metals). Biocatalysts are well known for their enzymatic activity towards numerous reactions ranging from in-vivo living cells (biochemical pathways) to in-vitro chemical reactions (reduction and transesterification) with enantiomeric purity and specificity. In this review the discussion is about synthesis of chiral using different kinds of biocatalysts. Daucus Carota, Pisum Sativa, Novazyme P-435, as biocatalysts for chiral alcohols.