Currently, accepted treatments for Female Sexual Dysfunction (FSD) are limited to psychological, behavioral, hormonal and psychopharmacologic interventions. Because of the complex and multifactorial nature of FSD, current therapeutic options may leave a subset of women suffering with sexual dysfunction without clinical improvement. As a simple, safe, and natural alternative therapeutic option for treating female sexual dysfunction, a pilot study was undertaken to test the effect, if any, of vaginal and clitoral injections of autologous Platelet Rich Plasma (PRP) on women desiring treatment for painful intercourse or anorgasmia. Two standardized sexuality tests, the Female Sexual Function Index and the Female Sexual Distress Scale, were administered before and after treatment and were used to measure the response to this therapeutic intervention. Our data indicated some degree of improvement in FSD, including positive changes in isolated sexual difficulties and in the reduction of levels of sexual distress. However, the limited number of participants in this pilot study restricts conclusions. Our initial observations do suggest that further investigation of PRP therapy for the treatment of female sexual dysfunction is indicated.