Current Synthetic and Systems Biology

Current Synthetic and Systems Biology
Open Access

ISSN: 2332-0737

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Gene Synthesis

Genes are a stretch of nucleotides which codes for a single polypeptide sequence. Genes are isolated and amplified artificially by PCR with gene specific primers if the sequence is known and sequenced. The desired sequence is synthesized artificially by solid phase DNA synthesis. Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences.

It is possible to make synthetic double stranded DNA molecule with no size limits. Oligonucleotides are synthesized by phosphoramidite nucleosides artificially. The used nucleosides can be natural or artificial. In 2010 mycoplasma genome was successfully synthesized artificially

Related Journals of Gene SynthesiS

Journal of Thermodynamics & Catalysis, Human Genetics & Embryology, Journal of Clinical Research & Bioethics, Clinical & Experimental Pharmacology, Gene Technology, Advances in Genetics, American Journal of Human Genetics, Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, Biochemical Genetics, Behavior Genetics, Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

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