Cell & Developmental Biology
Open Access

Role of Cytopathology in Detecting Disease at the Cellular Level

Kei La Toriah^{* *}Correspondence: Kei La Toriah, Department of Pharmacy Practice, Universitas Airlangga, Surabaya, Indonesia, Email:

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Description

Cytopathology is a medical specialty that focuses on the study and analysis of individual cells to diagnose diseases and understand cellular abnormalities. This field plays a significant role in early disease detection, guiding treatment decisions, and monitoring the progression of various medical conditions. It involves the microscopic examination of individual cells or small groups of cells obtained from various tissues and bodily fluids. These cell samples are collected through techniques such as fineneedle aspiration, Pap smears, bronchial washings, and body fluid analyses. In the era of personalized or precision medicine, cytopathology is integral to tailoring treatments to an individual's specific disease characteristics. It provides detailed information about the cellular and molecular properties of a patient's condition, enabling personalized treatment plans [1].

Common disease conditions of a cell

Cancer: Cancer is characterized by the uncontrolled growth and division of abnormal cells. These cells form tumors that can invade nearby tissues and spread to other parts of the body. There are many types of cancer, including breast cancer, lung cancer, and leukemia, each originating from different cell types.

Diabetes: Diabetes is a metabolic disorder that affects how cells use glucose (sugar) for energy. In type 2 diabetes, cells become resistant to the effects of insulin, leading to elevated blood sugar levels [2].

Neurodegenerative diseases: Diseases like Alzheimer's, Parkinson's, and Huntington's are characterized by the progressive death and dysfunction of nerve cells. These diseases often involve the accumulation of abnormal proteins within cells [3].

Muscular dystrophy: Muscular dystrophy refers to a group of genetic disorders that lead to the degeneration of muscle cells, resulting in muscle weakness and wasting. Different types of muscular dystrophy are caused by mutations in various genes [4].

Hemoglobinopathies: Sickle cell anemia and thalassemia are hemoglobinopathies that affect red blood cells. These conditions result from genetic mutations that lead to abnormal hemoglobin production, causing misshapen red blood cells and reduced oxygen-carrying capacity [5].

Autoimmune diseases: Autoimmune diseases, such as rheumatoid arthritis and multiple sclerosis, occur when the immune system mistakenly attacks the body's own cells [6].

Infectious diseases: Many infectious diseases, like HIV/AIDS, are caused by pathogens that invade and disrupt normal cellular function. Viruses, for instance, hijack host cell machinery to replicate.

Mitochondrial disorders: Mitochondrial diseases are caused by mutations in the DNA of mitochondria, the cell's energyproducing organelles. These conditions can lead to a lack of energy production and affect various organs and tissues [7].

Genetic disorders: Numerous genetic disorders, such as Down syndrome, cystic fibrosis, and Phenylketonuria (PKU), are caused by inherited mutations in specific genes that affect cellular processes.

Pap smear in cytopathology

In a Pap smear, a sample of cervical cells is collected and examined under a microscope. The cytopathologist looks for cellular abnormalities, such as changes in the size, shape, and structure of cervical cells. These may include enlarged nuclei, irregular cell borders, or increased nuclear-to-cytoplasmic ratio. The presence of abnormal cervical cells may indicate precancerous or cancerous conditions. For example, Human Papillomavirus (HPV) infections can lead to cellular changes that may progress to cervical cancer [8].

From a cell biology perspective, cytopathology provides insights into the molecular and cellular processes that underlie the development of cervical cancer. For instance, specific cellular changes may be associated with oncogenes and tumor suppressor genes. The primary benefit of Pap smears and cytopathology is early disease detection. By identifying cellular abnormalities, it is possible to catch precancerous or cancerous conditions at a stage when they are more treatable and have a higher chance of cure [9].

The results of cytopathology can inform treatment decisions. For example, if precancerous cells are detected, healthcare providers can recommend interventions to prevent the progression to invasive cancer. For individuals with abnormal results, follow-up Pap smears are often recommended to monitor the progression or regression of cellular abnormalities, emphasizing the importance of cellular surveillance [10].

Conclusion

These diseases highlight the diverse ways in which cellular abnormalities can lead to a wide range of health conditions. Understanding cellular biology and the underlying mechanisms of these diseases is essential for developing effective treatments and therapies. Intersection of pathology and cell biology, focusing on the microscopic analysis of individual cells to diagnose diseases, guide treatment, and further our understanding of various medical conditions. It is an essential component of modern medicine, with applications ranging from early cancer detection to the study of cellular changes in infectious diseases.

References

1. Frable WJ. Fine-needle aspiration biopsy: A review. Hum Pathol. 1983; 14(1):9-28. 

   [Crossref] [PubMed] [Google Scholar]

2. Frable WJ. Integration of surgical and cytopathology: A historical perspective. Diagn Cytopathol. 1995; 13(5):375-378. 

   [Crossref] [PubMed] [Google Scholar]

3. Martin HE, Ellis EB. Biopsy by needle puncture and aspiration. Ann Surg. 1930; 92(2): 169–181. 

   [Crossref] [PubMed] [Google Scholar]

4. Bousamra M, 2nd, Clowry L., Jr Thoracoscopic fine-needle aspiration of solitary pulmonary nodules. Ann Thorac Surg. 1997; 64(4):1191-1193

   [Crossref]  [PubMed] [Google Scholar]

5. Bardales RH, Stanley MW. Subcutaneous masses of the scalp and forehead: Diagnosis by fine-needle aspiration. Diagn Cytopathol. 1995; 12(2):131-134. 

   [Crossref] [PubMed] [Google Scholar]

6. Ryan AG, Zamvar V, Roberts SA. Iatrogenic candidal infection of a mediastinal foregut cyst following endoscopic ultrasound-guided fine-needle aspiration. Endoscopy. 2002; 34(10): 838-839. 

   [Crossref] [PubMed] [Google Scholar]

7. Kramer H, Groen HJ. Current concepts in the mediastinal lymph node staging of nonsmall cell lung cancer. Ann Surg. 2003; 238(2):180-188. 

   [Crossref] [PubMed] [Google Scholar]

8. Nasuti JF, Gupta PK, Baloch ZW. Diagnostic value and cost-effectiveness of on-site evaluation of fine-needle aspiration specimens: Review of 5,688 cases. Diagn Cytopathol. 2002; 27(1):1-4. 

   [Crossref] [PubMed] [Google Scholar]

9. Brown LA, Coghill SB. Cost effectiveness of a fine needle aspiration clinic. Cytopathology. 1992; 3(5):275-280. 

   [Crossref] [PubMed] [Google Scholar]

10. Dajani YF, Kilani Z. Role of testicular fine needle aspiration in the diagnosis of azoospermia. Int J Androl. 1998; 21(5):295-300. 

    [Crossref] [PubMed] [Google Scholar]