Multidimensional Separation Using HILIC and SCX Pre-fractionation for RP LC-MS/MS Platform with Automated Exclusion List-based MS Data Acquisition with Increased Protein Quantification

Yu Zhou^1,2, Zhen Meng⁴, Maria Edman-Woolcott⁴, Sarah F Hamm-Alvarez^3,4 and Ebrahim Zandi^1,2*

¹USC Research Center for Liver Diseases, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

²Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

³Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

⁴Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Ave, Los Angeles, California, USA

^*Corresponding Author: Ebrahim Zandi, University of Southern California, Norris Cancer Comprehensive Cancer Center, Keck School Of Medicine, Los Angeles, California, USA, Tel: (323)865-0644