Broad Utility of an Affinity-enrichment Strategy for Unanchored Polyubiquitin Chains

Jo Strachan¹, Barry Shaw¹, Varun Gopala Krishna¹, Daniel Scott¹, Juan C Del Pozo², Kristine Hill³, Madhav Gautam⁴, Dorota Skowyra⁴, Andrew D. Jacobson⁵, Chang-Wei Liu⁵, Neil J Oldham⁶ and Rob Layfield^1*

¹School of Life Sciences, University of Nottingham, Nottingham, UK

²Centro de Biotecnología y Genómica de Plantas INIA-UPM, Madrid, Spain

³Centre for Plant Integrative Biology, University of Nottingham, Nottingham, UK

⁴Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, Saint Louis, Missouri, USA

⁵Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado, USA

⁶School of Chemistry, University of Nottingham, Nottingham, UK

^*Corresponding Author: Rob Layfield, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom, Tel: +44-115-8230107