Advanced Techniques in Biology & Medicine
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Biology- Advanced Techniques

Suhasini Salivendra^{* *}Correspondence: Suhasini Salivendra, Departments of Biotechnology, Bhopal University, India, Email:

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Now a day’s studies in technological know-how is superior and properly evolved, new strategies and strategies have been evolved which helped within side the observe of shape of diverse forms of organisms and their cells. Microscope discovered to observe the minute organisms and additionally minute info of inner shape of organisms and cells. In the observe of biology, diverse new strategies and strategies have evolved, like microscopy, liquid, chromatography, distillation strategies and paper chromatography.

Simple Microscopes are not unusualplace technique the usage of to observe the microorganisms. Simple microscope is split into sorts primarily based totally at the lens used. One is Hand lens, it includes a biconvex lens, hooked up on a handle. This form of lens arecommonly used to exaggerate a whole item. Second one is Dissecting microscope; it includes a biconvex lens that's moved up and down through an screw attached, to deliver the item in sharp focus. A complete item may be visible via it.

Compound Microscopes are normally used withinside the biology laboratories to view small length or minute organisms and elements and sections of large organisms. Autoradiography is a way is used for observe the place of molecules and to hint metabolic activities withinside the cells. The radiolabelled compounds used for this method and those radiolabelled compounds will injected into the organism. Different sorts of tissues are investigated to discover wherein the radioactivity is located. This is carried out through the usage of photosensitive movie of silver bromide. Whenever withinside the mobileular or tissue or the organism, the radio labelled substance is present, silver receives decreased through radiation and is visible as black patches withinside the autoradiographs.

Cytochemical Methods is properly evolved and this technique is the usage of used to unique chemical components withinside the cells through differentiating a selected component from different elements with a selected stain or dye. Staining is carried out both through the usage of sure dyes or through the usage of the substrates of enzymes e.g. Schiff’s reagent, Feulgen staining, is used to localize the presence of DNA in a mobileular.

Paper Chromatography is any other technique withinside the discipline of biology and it's miles widely recognized technique. In this technique the chemical materials separated in combination. A small drop of the combination is placed on one quit of an extended strip of the Whatman clear out out paper. The clear out out paper quit with the drop of the combination dips into the solvent combination stored withinside the big tray. As the end result liquid is drawn up at the clear out out paper. According to their molecular weight, length one of a kind materials withinside the combination start to separate. The end result might be analysed through the usage of specificchemicals for similarly investigation.

Blotting method is superior method withinside the discipline of molecular biology. This form of strategies used to observe molecules such as DNA, RNA and proteins. Based at the observe of the molecule, blotting strategies are different sorts like southern, western and northerly blotting. Blotting method is carried out through combination of DNA, RNA or protein go with the drift via a slab agarose or polyacrylamide gel. This agarose gel permits small molecules to transport quicker than larger ones. The separated molecules are then pressed in opposition to a membrane, which enables pass the molecules from the gel onto the membrane.