Amplicon Secondary Structure Formation and Elongation during the Process of Sequencing

Elham Davoudi-Dehaghani^1,2, Sirous Zeinali^2,3*, Nejat Mahdieh^3,4, Atefeh Shirkavand⁵ and Hamideh Bagherian³

¹Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

²Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur St., Tehran, Iran

³Medical Genetics Lab, Kawsar Human Genetics Research Center, No. 41 Majlesi St., Vali Asr St., Tehran, Iran

⁴Growth and Development Research Center, Tehran University of Medical Sciences, Iran

⁵Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran

^*Corresponding Author: Sirous Zeinali, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute Of Iran, Pasteur St., Tehran, Postal Code: 1595645513, Iran, Tel: 989121372040, Fax: 98 21 88939140