A Method for Isolation and Proteomic Analysis of Outer Membrane Vesicles from Fecal Samples by LC-MS/MS

Jing Wu¹, Mingrui An¹, Jianhui Zhu¹, Zhijing Tan¹, Grace Y. Chen², Ryan W Stidham^3,4 and David M. Lubman^1*

¹Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA

²Department of Internal Medicine, Division of Hematology/Oncology University of Michigan, Ann Arbor, MI 48109, USA

³Department of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, 48109, USA

⁴Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA

^*Corresponding Author: David M. Lubman, Department of Surgery, University of Michigan Medical Center, 1150 West Medical Center Drive, Building MSRB1, Rm A510B, Ann Arbor, MI 48109-0656, United States, Tel: + (734)-615-5081, Fax: + (734)-615-2088