A Chloroform-Assisted Protein Isolation Method Followed by Capillary NanoLC-MS Identify Estrogen-Regulated Proteins from MCF7 Cells

Adaikkalam Vellaichamy¹, Chin-Yo Lin¹, Thin Thin Aye¹, Govindarajan R. Kunde¹, Alexey I. Nesvizhskii², Edison T. Liu¹ and Siu Kwan Sze^1*

¹Genome Institute of Singapore, 60 Biopolis Street, 138672, Singapore

²Department of Pathology, University of Michigan, MI 48109, USA

^*Corresponding Author: Siu Kwan Sze, PhD, Assistant Professor, School of Biological Sciences, Nanyang Technological University, 637551, Singapore, Tel: +65-6316-2852, Fax: +65-6791-3856