Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
ISSN: 2157-7013
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA
Research Article
Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections
Author(s): Oluwaseyi Awonusi, Zachary J. Harbin, Sarah Brookes, Lujuan Zhang, Samuel Kaefer, Rachel A. Morrison, Sharlé Newman, Sherry Voytik-Harbin and Stacey Halum*
Objective: To describe how differing injector needles and delivery vehicles impact Autologous Muscle-Derived Cell
(AMDC) viability when used for laryngeal injection.
Methods: In this study, adult porcine muscle tissue was harvested and used to create AMDC populations. While
controlling cell concentration (1 × 107 cells/ml), AMDCs including Muscle Progenitor Cells (MPCs) or Motor
Endplate Expressing Cells (MEEs) were suspended in either phosphate-buffered saline or polymerizable (in-situ
scaffold forming) type I oligomeric collagen solution. Cell suspensions were then injected through 23- and 27-gauge
needles of different lengths at the same rate (2 ml/min) using a syringe pump. Cell viability was measured immediately
after injection and 24- and 48-hours post-injection, and then compared to baseline cell viabi.. View More»
DOI:
10.35248/2157-7013.22.14.377