Journal of Stem Cell Research & Therapy

Yu-Yu Joyce Ho

Publications

• Research Article
  Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants
  Author(s): Li-Yi Sun, Dian-Kun Li, Pao-Jen Chen, Yu-Yu Joyce Ho, Jon-Son Kuo, Ching-Feng Cheng, Kuei-Fang Lee, Yao-Jen Chang and Cheng-Yoong PangLi-Yi Sun, Dian-Kun Li, Pao-Jen Chen, Yu-Yu Joyce Ho, Jon-Son Kuo, Ching-Feng Cheng, Kuei-Fang Lee, Yao-Jen Chang and Cheng-Yoong Pang
  
  Objective: Excised fat tissue (EFT) is an excellent source of adipose-derived stem cells (ADSCs) for autologous cell therapy. However, the current technique for excising fat tissues with conventional surgical scissors and using culture medium containing fetal bovine serum (FBS) may not comply with Good Tissue Practice (GTP) guidelines. We thus developed a semi-automated process complying with the abovementioned guideline to manufacture clinicalgrade ADSCs from small volumes of EFT. Methods: A semi-automated process was developed by using a disposable disperser tube operated on a Tube Disperser Workstation to obtain stromal vascular fraction (SVF) from EFT. A culture medium supplemented with human autologous serum (HAS) and mesenchymal stem cell culture adjuvant (MCA) consisted of basic fibroblast growth factor (bFGF/FGF-2) and antioxidants, was formulated to expand ADSCs from SVF in v.. View More»
  DOI: 10.4172/2157-7633.1000193

  Abstract PDF