International Journal of Waste Resources

Yuta Kato

Publications

• Research Article
  Newly-Isolated Laccase High Productivity Streptomyces Sp. Grown In Cedar Powder as the Sole Carbon Source
  Author(s): Akihisa Aoyama, Kazuhiro Yamada, Yoshinobu Suzuki, Yuta Kato, Kazuo Nagai and Ryuichiro KuraneAkihisa Aoyama, Kazuhiro Yamada, Yoshinobu Suzuki, Yuta Kato, Kazuo Nagai and Ryuichiro Kurane
  
  Microorganisms with greater potential to degrade lignin than well-known white-rot fungi were sought and identified, but the fungi were not less frequently employed for slow growth and little enzyme productivity. They were subjected to enriched cultures in order to explore the bacteria instead from 300 soil samples with cedar powder as the sole carbon source were prepared. From these, a culture with actinomycetes which showed the most oxidation activity of 2,6-Dimetoxyphenol (2,6-DMP) known as laccase substrate was selected and labeled as KS1025A strain. Characteristics of the bacteria and behavior of the secreted enzymes were examined. As a result, it was identified as a strain of Streptomyces sp. from the 16S rDNA gene sequence homology. The optimum temperature and pH for laccase activity of the secreted enzyme of this strain are 50°C and 4.5, respectively. Since Mn2+ was not dir.. View More»
  DOI: 10.4172/2252-5211.1000146

  Abstract PDF