Biochemistry & Analytical Biochemistry

Vladimir Tolstikov

Publications

• Research Article
  Blood Sampled Through Dried Blood Spots (DBS) Exhibits Diminished Ex vivo Metabolism Compared to Whole Blood Through Use of a Kinetic Isotope-Labeling Metabolomics Approach
  Author(s): Collin Hill, Jeremy Drolet, Mark D Kellogg, Vladimir Tolstikov, Niven R Narain and Michael A KiebishCollin Hill, Jeremy Drolet, Mark D Kellogg, Vladimir Tolstikov, Niven R Narain and Michael A Kiebish
  
  Blood is the primary matrix for metabolite profiling, providing a means for biomarker identification, pharmacokinetic/ pharmacodynamic analysis and disease monitoring. Conventional methodologies of blood sample collection require blood drawn by venous puncture. However, this technique allows for residual ex vivo metabolic activity of the blood matrix, thus presenting a challenge to capturing a physiologically representative readout of the metabolome. Blood that is not immediately processed is subjected to extended periods of ex vivo metabolism. Even when samples are transported by cold storage, some enzymatic processes remain active. The dried blood spot (DBS) collection technique renders cells metabolically inactive in a short span of time. We demonstrate that whole blood deposited onto a DBS card decreases uptake and metabolism of U13C-glucose after 4 hours, as analyzed by mass spec.. View More»
  DOI: 10.4172/2161-1009.1000325

  Abstract PDF