Uzma Noor Shah
India
Research Article
Genetic Diversity Analysis of Walnut (Juglans regia L.) from Kashmir Valley Using RAPD and ISSR Markers
Author(s): Uzma Noor Shah, JI Mir, N Ahmed and Khalid M FaziliUzma Noor Shah, JI Mir, N Ahmed and Khalid M Fazili
Walnut (Juglans regia) is one of the most essential nutritive and economic nut crops. Genetic diversity analysis of 96 walnut genotypes from Kashmir was done using 13 RAPD and 19 ISSR markers. RAPD diversity analysis revealed 27.78% polymorphism while as ISSR analysis showed 93.05% of polymorphic bands. Jaccards similarity co-efficient ranged from 0.78 to 1 with an average of 0.91 for RAPD and 0.22 to 0.85 with an average of 0.58 for ISSR markers and 0.59 to 0.92 with an average of 0.74 for combined data. Cluster analysis using UPGMA by RAPD and ISSR markers obviously revealed distinct diversity between genotypes. The dendrogram constructed from the combination of RAPD and ISSR markers categorized the 96 genotypes into one major cluster and five minor clusters. Effective number of alleles (Ne), Nei’s gene diversity (H) and Shannon’s In.. View More»
DOI:
10.35248/2168-9881.19.8.185