Tim R. Nagy
Tanzania
Research Article
Carnitine Palmitoyltransferase 1b Deficiency Protects Mice from Diet-Induced Insulin Resistance
Author(s): Teayoun Kim, Lan He, Maria S. Johnson, Yan LI, Ling Zeng, Yishu Ding, Qinqiang Long, John F. Moore, Jon D. Sharer, Tim R. Nagy, Martin E. Young, Philip A. Wood and Qinglin YangTeayoun Kim, Lan He, Maria S. Johnson, Yan LI, Ling Zeng, Yishu Ding, Qinqiang Long, John F. Moore, Jon D. Sharer, Tim R. Nagy, Martin E. Young, Philip A. Wood and Qinglin Yang
Background: Carnitine Palmitoyl Transferase 1 (CPT1) is the rate-limiting enzyme governing long-chain fatty acid entry into mitochondria. CPT1 inhibitors have been developed and exhibited beneficial effects against type II diabetes in short-term preclinical animal studies. However, the long-term effects of treatment remain unclear and potential non-specific effects of these CPT1 inhibitors hamper in-depth understanding of the potential molecular mechanisms involved.
Methods: We investigated the effects of restricting the activity of the muscle isoform CPT1b in mice using heterozygous CPT1b deficient (Cpt1b+/-) and Wild Type (WT) mice fed with a High Fat Diet (HFD) for 22 weeks. Insulin sensitivity was assessed using Glucose Tolerance Test (GTT), insulin tolerance test and hyperinsulinemic euglycemic clamps. We also examined body weight/composition, tissue and systemic .. View More»
