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Shilpy Sharma
Shilpy Sharma
Tanzania
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Research Article
Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria
Author(s): Rengathavasi Thavasi, Shilpy Sharma and Singaram JayalakshmiRengathavasi Thavasi, Shilpy Sharma and Singaram Jayalakshmi
Hemolytic assay, bacterial adhesion to hydrocarbons (BATH) assay, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing bacteria. 105 bacterial strains were screened for their biosurfactant production. Results from screening methods revealed that hemolytic and BATH assays are not reliable methods to check biosurfactant production because hemolytic assay relies on lysis of blood cells, which can be caused by compounds produced by microbes other than the biosurfactants. In that case hemolytic assay may include strains that do not produce biosurfactant. BATH assay relies on hydrophobicity or adherence of cells with hydrophobic compounds. Results obtained in BATH assay for three bacterial isolates, Branhamella catarrhalis, Citrobacter intermedius and Klebsiella oz.. View More»
DOI: 10.4172/2157-7463.S1-001