Saqib Mahmood
Pakistan
Research Article
Hyper-production of Alkaline Protease by Mutagenic Treatment
of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged
Fermentation
Author(s): Sadia Javed, Munazzah Meraj, Shazia Anwer Bukhari, Rao Irfan and Saqib MahmoodSadia Javed, Munazzah Meraj, Shazia Anwer Bukhari, Rao Irfan and Saqib Mahmood
Green chemistry technologies are the powerful tool for the management of environmental wastes challenges. Agro industrial residues are composed of complex polysaccharides that support the microbial growth for the production of useful products (enzymes, organic acids, drugs, etc.). Disposal and environment friendly management of these wastes has become a global priority. The aim of present investigation was to improve the alkaline protease yield by treating the parent Bacillus subtilis M-9 strain with different mutagens UV-irradiations, N-methyl-N-nitro- N-nitrosoguinidine (NTG), Ethidium bromide (EB), using agroindustrial wastes (banana stalk and corn stover) in submerged fermentation. Fifteen positive mutants were selected on skim milk agar plates for shake flask experiments. BSU-5 mutant strain showed 81.21± 3.24 PU/mL alkaline protease activity higher than parent strain (23... View More»
DOI:
10.4172/1948-5948.1000103