Fermentation Technology

Roberto da Silva

Publications

• Research Article
  Production of β-(1,3)-glucanases by Trichoderma harzianum Rifai: Optimization and Application to Produce Gluco-oligosaccharides from Paramylon and Pustulan
  Author(s): Ellen C. Giese, Robert F. H. Dekker, Aneli M. Barbosa, Maria de Lourdes Corradi da Silva and Roberto da SilvaEllen C. Giese, Robert F. H. Dekker, Aneli M. Barbosa, Maria de Lourdes Corradi da Silva and Roberto da Silva
  
  β-(1→3)-Glucanases were produced by Trichoderma harzianum Rifai PAMB-86 cultivated on botryosphaeran in a bench-fermenter and optimised by the response surface method. Maximal enzyme titres occurred at 5 days, initial pH 5.5 and aeration of 1.5vvm. β-(1→3)-The β-glucanolytic enzyme complex produced by T. harzianum Rifai PAMB- 86 was fractionated by gel filtration into 2 fractions (F-I, F-II), and employed to produce gluco-oligosaccharides from algal paramylon ((1→3)-β-D-glucan) and lichen pustulan ((1→6)-β-D-glucan). Both enzymes attacked paramylon to the extent of ~15-20% in 30 min releasing glucose and laminaribiose as major end-products, and laminari- oligosaccharides of degree of polymerization (DP) ≥ 3. Only F-I degraded pustulan resulting in ~2% degradation at 30 min, with glucose, gentiobiose and.. View More»
  DOI: 10.4172/2167-7972.1000102

  Abstract PDF