Biochemistry & Analytical Biochemistry

Rajamanickam V

Publications

• Research Article
  A Generic Workflow for Bioprocess Analytical Data: Screening Alignment Techniques and Analyzing their Effects on Multivariate Modeling
  Author(s): Rajamanickam V, Herwig C and Spadiut ORajamanickam V, Herwig C and Spadiut O
  
  UV chromatographic data in combination with multivariate data analysis (MVDA) has been extensively used for bioprocess monitoring. However, they are usually attributed to shifts along the retention time and require preprocessing. Misaligned UV chromatographic data result in inconsistent MVDA models. Numerous preprocessing techniques are available, each varying in the number of meta-parameters to optimize, complexity and computational time. Therefore, we aimed at developing a generic workflow to screen for preprocessing techniques. We chose four datasets with increasing complexity containing UV chromatographic data from reverse-phase and size exclusion chromatography HPLC. We aligned all four datasets using three preprocessing techniques, namely icoshift, PAFFT and RAFFT algorithms. We chose several statistical tools to validate the performance of the preprocessing techniques and to sc.. View More»
  DOI: 10.35248/2161-1009.19.8.373

  Abstract PDF