Nourkhoda Sadeghifard
West Indies
Research Article
Extended Spectrum Beta-Lactamases Among Hospitalized Patients in Surgery Wards, Ilam, Iran
Author(s): Nourkhoda Sadeghifard, Sobhan Ghafourian, Zamberi bin Sekawi, Vasantha Kumari Neela, Ali Hematian, Iraj Pakzad, Elham Abouali Galehdari and Reza MohebiNourkhoda Sadeghifard, Sobhan Ghafourian, Zamberi bin Sekawi, Vasantha Kumari Neela, Ali Hematian, Iraj Pakzad, Elham Abouali Galehdari and Reza Mohebi
Objectives of this study were to study the molecular epidemiology of ESBL-producing Klebsiella pneumoniae in selective hospitals in Iran, to determine the prevalence of TEM, SHV and CTX-M genes responsible for ESBL production among ESBL-producing K. pneumoniae, to investigate the susceptibility of Klebsiellae spp producing ESBLs towards non-beta-lactam antibiotics, all in different seasons. Clinical isolates of K. pneumoniae were identified during Mar. 2007 to Apr. 2008 in Ilam hospitals, the province in west of Iran. All isolates were found in surgery wards. ESBL activity was first evaluated using the standard disc diffusion test for cephalosporins and monobactam, then using the double-disc synergy test between cephalosporins and clavulanate. PCR assay had done for ESBLs genes detection. The results showed sixteen K.pneumoniae were identified by chemical methods. No resistance had oc.. View More»
DOI:
10.4172/1948-5948.1000044