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Luiz Augusto Basso
Luiz Augusto Basso
Centro de Pesquisas em Biologia, Molecular e Funcional (CPBMF), Instituto Nacional de Ciencia e Tecnologia em, Tuberculose (INCT-TB), Pontifícia Universidade Católica do Rio Grande do, ul (PUCRS), Av. Ipiranga, 6681, Tecnopuc, Prédio ,
Brazil
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Research Article
Comparison between Two Erwinia carotovora L-Asparaginase II Constructions: cloning, Heterologous Expression, Purification, and Kinetic Characterization
Author(s): Priscila Lamb Wink, Heique Marlis Bogdawa, Gaby Renard, Jocelei Maria Chies, Luiz Augusto Basso and Diógenes Santiago SantosPriscila Lamb Wink, Heique Marlis Bogdawa, Gaby Renard, Jocelei Maria Chies, Luiz Augusto Basso and Diógenes Santiago Santos
L-Asparaginase II from Erwinia carotovora may represent an important alternative therapy in the treatment of acute childhood lymphoblastic leukaemia, despite its promising lower glutaminase activity than Escherichia coli and Erwinia chrysanthemi L-asparaginases II, currently used in treatment of this disease. Here we describe cloning, expression, purification and determination of steady-state kinetic parameters for E. carotovora L-asparaginase II: with (AspSP) and without the signal peptide (AspMP). AspMP was purified to homogeneity by a single-step protocol with 91% yield, and AspSP by a two-step protocol with 28% yield. In addition, both enzymes presented similar high specific activities: 208.1 and 237.6 U mg-1, respectively. The Km and kcat values showed that AspMP has lower glutaminase activity than AspSP. Moreover AspMP is produced by a sim.. View More»