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Kalpana Mody
Kalpana Mody
Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar 364 002, Gujarat,
India
Publications
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Research Article
Characterization of Alkaliphilic, Surfactant Stable and Raw Starch Digesting A-Amylase from Bacillus subtilis Strain JS-16
Author(s): Gopalakrishnan Menon, Kalpana Mody, Sumitra Datta and Bhavanath JhaGopalakrishnan Menon, Kalpana Mody, Sumitra Datta and Bhavanath Jha
An alkali-thermophilic and surfactant stable α-amylase was obtained from Bacillus subtilis strain JS-16, isolated from sludge samples of a soda ash industry, located on west coast of Gujarat. It was active over a wide temperature range (30°C-80°C) exhibiting optimum activity at 50°C and pH 9.0. Unique feature of this amylase was two-fold enhancement of activity in SDS. Three-step purification yielded 15.16-fold purified enzyme with specific activity of 13.5 U/mg proteins. Km and Vmax were 10 mg/ml and 0.2 μmol/min/ml (11.56 μmol/ min/mg protein) respectively. Enzyme activity enhanced with Fe3+ but was strongly inhibited by Hg2+ ions. The amylase hydrolyzed 12% raw wheat starch and 5% corn starch granules after 12 h incubation. Surfactant stability, alkaliphilic nature, activity under wide temperature range and hydrolyses of raw starch makes this amylase a promi.. View More»
DOI: 10.4172/1948-5948.S8-002