K Suribabu
PG Department of Microbiology and Research Centre, Dr. Lankapalli Bullayya Post-graduate College, Visakhapatnam-530 013, A.P,
India
Research Article
Strain Improvement of Brevibacillus borostelensis R1 for Optimization of
α-Amylase Production by Mutagens
Author(s): K Suribabu, T Lalitha Govardhan and KPJ HemalathaK Suribabu, T Lalitha Govardhan and KPJ Hemalatha
Physical and chemical mutagens are promising and are used for screening of high yielding strains. The exponential increase in the application of amylases in various fields has placed stress and demand in both qualitative improvement and quantitative enhancement through strain improvement. Ultraviolet light exerts its mutagenic effect by exciting electrons in molecules. The potent UV mutants which showed more than 20 mm zone of starch hydrolysis were screened and selected at 42% of survival time at 80minutes of exposure. The wild strain with fixed parameters yielded (3000 U/ml). The major findings of the strain improvement were out of ten mutants isolated, two (UV-3 and UV-10) showed 3000-4000 U/ml of amylase activity. The % of survival of Brevibacillus borstelensis R1 in Pikovskaya’s medium was 25.75% at 120 minutes of exposure. Ten mutants (HNO2-10, HNO2-30, EMS-4, EtBr-40, EtB.. View More»
DOI:
10.4172/1948-5948.1000132