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Jyoti P Jadhav
Jyoti P Jadhav
Professor and Head, Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004,
India
Publications
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Research Article
Single Cell Protein Production Using Penicillium ochrochloron Chitinase and Its Evaluation in Fish Meal Formulations
Author(s): Nilambari S Patil and Jyoti P JadhavNilambari S Patil and Jyoti P Jadhav
The conversion of the enzymatic hydrolysate of shellfish chitin to single-cell protein was investigated. The endproduct of chitin hydrolysis by Penicillium ochrochloron chitinase was mainly N-acetyl-D-glucosamine, its further utilization as a substrate for SCP production using Yerrowia lipolytica NCIM 3450 has been studied. The 2% chitin hydrolysate was found to be optimal for SCP production. A biomass of 9.4 g/l, total protein and nucleic acid content of the biomass was 65% and 2.9%, respectively. Fish diets were formulated to replace fishmeal partially by single cell protein from Yerrowia lipolytica using chitin hydrolysate in diets of Lepidocephalus thermalis for a period of 2 weeks. Control diet and three experimental diets were prepared to replace 25%, 50% and 75% of fish meal using SCP. The result indicated 50% yeast SCP diet gave the better growth response in Lepidocephalus the.. View More»
DOI: 10.4172/1948-5948.S4-005