Pharmaceutica Analytica Acta

Hua Li

Publications

• Research Article
  Simultaneous Quantitative Determination of Nine Bufadienolides in Traditional Chinese Medicinal Toad Skin from Different Regions of China by High-Performance Liquid Chromatography–Photodiode Array Detection
  Author(s): Si-Wang Wang, Lin-Rui Duan, Wei Cao, Yan-Hua Xie, Jia-Ni Yuan, Hua Li and Xiao-Kai ZhangSi-Wang Wang, Lin-Rui Duan, Wei Cao, Yan-Hua Xie, Jia-Ni Yuan, Hua Li and Xiao-Kai Zhang
  
  Toad skin is a traditional Chinese medicine for the treatment of various tumors. The major active components in toad skin are bufadienolides. In this paper, a simple, accurate and reliable method for the simultaneous separation and determination of nine active components (gamabufotalin, arenobufagin, telocinobufagin, desacetylcinobufotalin, bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogeni) in toad skin was developed using High-Performance Liquid Chromatography (HPLC) coupled with Photodiode Array Detection (PDA) detection. The chromatographic separation was performed on a SinoChrom ODS-BP C18 column with gradient elution using acetonitrile and 0.1% acetic acid-0.5% potassium dihydrogen phosphate aqueous solution at a flow rate of 0.8 mL min-1. All compounds showed good linearity in a wide concentration range with the values of r2 higher.. View More»
  DOI: 10.4172/2153-2435.1000345

  Abstract PDF