Helmut Brath
Austria
Research Article
Abundance and Diversity of Microbiota in Type 2 Diabetes and Obesity
Author(s): Marlene Remely, Simone Dworzak, Berit Hippe, Jutta Zwielehner, Eva Aumüller, Helmut Brath and Alexander HaslbergerMarlene Remely, Simone Dworzak, Berit Hippe, Jutta Zwielehner, Eva Aumüller, Helmut Brath and Alexander Haslberger
Inflammatory reactions leading to the progression of metabolic syndrome contribute to changes in composition of the GIT microbiota.
We characterize fecal microbiota at three time points in fourteen obese participants, nineteen lean controls and twenty-four type 2 diabetes patients. Obese and type 2 diabetics received an intervention of nutritional counseling, type 2 diabetics an additional therapy with a GLP-1-Agonist.The microbiota composition was analyzed for abundance and diversity by quantitative real-time polymerase chain reaction, denaturing gradient gel electrophoresis and high throughput sequencing.
In type 2 diabetics an increase of diversity was observed with intervention whereas the values of lean controls remained unaffected. In the lean and obesity groups, a lower Firmicutes:Bacteroidetes ratio correlated with lower BMI. In type 2 diabetics the ratio o.. View More»
