Journal of Stem Cell Research & Therapy

Halff Glenn

Publications

• Research Article
  Chemical Induction of Human Adipose Stromal Cells Into Hepatocyte-Like Cells under Various Differentiation Conditions
  Author(s): Coronado Ramon, Somaraki-Cormier Maria, Natesan Shanmugasundaram, Christy Robert, Ong Joo and Halff GlennCoronado Ramon, Somaraki-Cormier Maria, Natesan Shanmugasundaram, Christy Robert, Ong Joo and Halff Glenn
  
  Background: A shortage of donor livers for transplant has led to an increased interest in cell therapeutics as an alternative to whole organ transplant to treat end-stage liver disease. Primary human hepatocytes have been used in cell-based therapies. However, hepatocytes do not proliferate in vitro so it is challenging to grow enough cells for a successful transplant. Many have suggested using hepatocyte-like Adipose-derived mesenchymal Stromal/ stem Cells (ASCs) differentiated into hepatocyte-like cells as a substitute. Here we evaluate how closely these cells resemble primary hepatocyte cell morphology and function.Methods: Human ASCs were mechanically isolated from lipoaspirates. The stem cell nature of ASCs was characterized using flow cytometry and tri-lineage differentiation into osteocytes, adipocytes, and chondrocytes. ASCs wer.. View More»
  DOI: 10.4172/2157-7633.1000424

  Abstract PDF