Journal of Bacteriology & Parasitology

George L. Mendz

Publications

• Research Article
  Identification of NuoX and NuoY Ligand Binding Specificity in the Campylobacter Jejuni Complex I
  Author(s): Lirio I. Calderon-Gomez, Christopher J. Day, Lauren E. Hartley-Tassell, Jennifer C. Wilson, George L. Mendz and Victoria KorolikLirio I. Calderon-Gomez, Christopher J. Day, Lauren E. Hartley-Tassell, Jennifer C. Wilson, George L. Mendz and Victoria Korolik
  
  The components of the proton pump NADH:ubiquinone (Complex I) of the respiration pathway have been identified in the C. jejuni genome. However, the paradigm genes nuoE and nuoF encoding subunits of the NADH dehydrogenase module of Complex I are absent. Instead the genes cj1575c and cj1574c encoding NuoX and NuoY are present in the loci corresponding to nuoE and nuoF, respectively. Bioinformatics analyses showed the presence of nuoX and nuoY homologues in all sequenced strains of C. jejuni and in other Campylobacter species, as well as the presence of orthologues in other É�-Proteobacteria. To understand the involvement of the NuoX and NuoY proteins in the respiration of C. jejuni and to characterize their ligand binding specificity and affinity, a tricarboxylic acid cycle array was developed as a tool to identify proteins that can bind to intermediates of this cycle as well as .. View More»
  DOI: 10.4172/2155-9597.1000307

  Abstract PDF