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George Baltopoulos
George Baltopoulos
Greece
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Research Article
Alcohol Effects on TNF-α and IL-10 Production in an Ex-Vivo Model of Whole Blood Stimulated by LPS
Author(s): Alexandra Gavala, Pavlos Myrianthefs, Kyriaki Venetsanou, George Baltopoulos and Georgios AlevizopoulosAlexandra Gavala, Pavlos Myrianthefs, Kyriaki Venetsanou, George Baltopoulos and Georgios Alevizopoulos
Alcohol exposure is related to increased susceptibility to infections. We investigated the effect of acute exposure to alcohol on pro- and anti-inflammatory cytokine production in an ex-vivo model of whole blood stimulation with lipopolysacharide (LPS). Ten ml of whole blood were taken from 24 healthy volunteers (all men) aged 36.5 ± 1.4 years. Each sample was transferred into two tubes, without and with EDTA as anticoagulant, under sterile conditions. We had 14 groups: the control group in which whole blood was incubated without any other intervention, the LPS group in which whole blood was stimulated with LPS alone, and 12 alcohol groups with (6 groups) and without LPS stimulation (6 groups) using six different doses of alcohol (5‚ 12.5‚ 25‚ 50‚ 100 and 200mM). LPS (500pg) was added after pretreatment with alcohol for 10 mi.. View More»
DOI: 10.4172/2378-5756.1000339