Farhad Moosakhani
West Indies
Research Article
The Relationship between Microscopic Lesions and Different Types of Clostridium perfringens and their Related Toxins by Sandwich ELISA in
Cattle
Author(s): Farhang Sasani, Havva Marzban, Javad Javanbakht, Farhad Moosakhani and Mahsa ImanparastFarhang Sasani, Havva Marzban, Javad Javanbakht, Farhad Moosakhani and Mahsa Imanparast
Obviously, one of the most important causes of Enterotoxaemia in domestic animals is Clostridium perfringens. In present study, C. perfringens type A (56.66%), type D (26.66%) and type B (16.66%) were respectively the most abundant bacteria in intestinal samples of 30 cattle and calves with enterotoxaemia. The incidence of α, β and ε toxins in extracted intestinal contents, by Sandwich ELISA method, were determined 89/98%, 99/19% and 33.33% respectively. Histopathologic examination of different organs from 12 cattle and calves with enterotoxaemia and C.perfringens affection indicated following lesions; Heart: congestion (40%), hemorrhage (30%), mild myolysis (10%), sarcocystosis (30%), lymphocytic endocarditis (10%), pericarditis (10%) and myocardiocyte hypertrophy (20%). Liver: hepatitis (27%), coagulative necrosis (36%), hemorrhage (19%), severe hepatic fibrosis (.. View More»
DOI:
10.4172/1948-5948.1000096