Clara Si Hua Tan
Singapore
Research Article
Adiponectin Gene Polymorphisms and Type 2 Diabetes among Singaporean Chinese Adults
Author(s): Wan Ching Toy, Jian-Jun Liu, Anton Kui Sing Cheng, Clara Si Hua Tan, Dawn Pingxi Lau, Melvin Deng Sheng Wong, Tavintharan Subramaniam, Chee Fang Sum and Su Chi LimWan Ching Toy, Jian-Jun Liu, Anton Kui Sing Cheng, Clara Si Hua Tan, Dawn Pingxi Lau, Melvin Deng Sheng Wong, Tavintharan Subramaniam, Chee Fang Sum and Su Chi Lim
Background: Adiponectin is the most abundant circulating adipokine in human that regulates insulin actions. Association of adiponectin gene variations with type 2 diabetes (T2DM) has been reported albeit predominantly in non-Asian populations. Additionally, proof of variant functionality beyond statistical association is often unavailable. We studied six common (minor allele frequency ?0.05) adiponectin single nucleotide polymorphisms (SNPs) in Singaporean Chinese adults with follow-up functional genetic experiments. Methods: In a case-control study (N=588), genotyping of six common adiponectin haplotype tagging SNPs [-3964A>G(rs822396), +45T>G(rs2241766), 276C>A(rs1501299), 973G>A(rs3774262), 4551G>C(rs1063539) and 5852G>A(rs6444175)] was performed using Taqman genotyping assay. Allele-dependent differential efficiency of mRNA expression was tested with quantitati.. View More»
