Journal of Blood Disorders & Transfusion

Anna V Chernatynskaya

Publications

• Research Article
  Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice
  Author(s): Ben M Looney, Anna V Chernatynskaya, Michael J Clare-Salzler and Chang-Qing XiaBen M Looney, Anna V Chernatynskaya, Michael J Clare-Salzler and Chang-Qing Xia
  
  Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expanded ex vivo with GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serumfree culture media (X-VIVO20; FBS-) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS- BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Th cell response induced by FBSBM- DC. Finally, we demonstrate that only FBS- BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+ regulatory T cells as well as a long-lasting β cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic.. View More»
  DOI: 10.4172/2155-9864.1000206

  Abstract PDF