Journal of Clinical & Experimental Pharmacology

Alsousi AA

Publications

• Research Article
  Cytokine-mediated Differential Regulation of Cyclooxygenase-2, High Mobility Group Box 1 Protein and Matrix Metalloproteinase-9 Expression in Fibroblast-like Synovial Cells
  Author(s): Alsousi AA, Siddiqui S and Igwe OJAlsousi AA, Siddiqui S and Igwe OJ
  
  Persistent joint inflammation and pain with concomitant joint erosion, characterize Rheumatoid Arthritis (RA). We used a Fibroblast-like Synovial (FLS) cell line derived from a female rabbit, as a model system for studying the initiation and attenuation of conditions of RA in vitro. We used two pro-inflammatory cytokines, TNFα and IL-1β to examine potential inflammatory responses and cartilage erosion exerted by each cytokine alone and/or in combination. We determined the expression levels of cytokine-induced expression of Cyclooxygenase-2 (COX-2), production of Prostaglandin E2 (PGE2), release of high mobility group box-1 (HMGB1) protein and the activity of Metalloproteinase-9 (MMP-9) in FLS cells. Treatment with TNFα alone increased HMGB1 release levels, MMP-9 activity, COX-2 expression and PGE2 production in both concentration- and exposure time-dependent .. View More»
  DOI: 10.4172/2161-1459.1000240

  Abstract PDF