Alberto Reis
Portugal
Research Article
Monitoring Rhodotorula glutinis CCMI 145 Stress Physiological Response during Fed-Batch Fermentations Using Multi-Parameter Flow Cytometry
Author(s): Teresa Lopes da Silva, Daniela Feijão and Alberto ReisTeresa Lopes da Silva, Daniela Feijão and Alberto Reis
Multi-parameter flow cytometry was used to monitor R. glutinis stress response during a fed-batch fermentation, through cell viability, lipid content and intrinsic light scatter. During the yeast fermentation, the proportion of cells with permeabilized membrane (dead cells) increased when nutrients and/or oxygen became limiting. Yeast cells showed a higher injury level when grown under other nutrient limitation than under oxygen limiting conditions, as the dead cells reduced their internal content and size in the former situation, suggesting drastic cells lysis.
The maximum yeast lipid content was 8% (w/w) at t=38.3 h. Such low lipid content was attributed to oxygen limitation, which highlights the importance of the oxygen transfer rate when producing lipids from aerobic yeast cultures.
Changes in Forward and Side scatter light .. View More»
DOI:
10.4172/1948-5948.1000042